EdU cell proliferation detection kit

EdU (5-ethynyl-2′-deoxyuridine), whose Chinese name is 5-ethynyl-2′-deoxyuridine, is a thymidine analogue. Its alkyne hydroxyl group is rare in natural compounds. When cells proliferate, they can replace thymidine (thymidine) and be inserted into the replicating DNA molecules. The ethynyl group on EdU can pass through with fluorescently labeled small molecule azide probes (such as Azide Alexa Fluor 488, etc.) Monovalent copper ions catalyze a covalent reaction to form a stable triazole ring. This reaction is very rapid and is called a click reaction, which allows efficient and rapid detection of cell proliferation, especially the effective detection of S The percentage of cells in the stage.
Compared with the traditional immunofluorescence staining (BrdU) detection method, EdU is only 1/500 of the size of the BrdU antibody, is easier to diffuse in cells, does not require strict sample denaturation (acid hydrolysis, pyrolysis, enzymatic hydrolysis), and is effective It effectively avoids sample damage and helps to observe the true situation of cell proliferation at the overall level of tissues and organs, with higher sensitivity and faster detection speed.